[FlashwithSymbols]

We did an experiment to estimate the isoelectric point of Casein by pH/Turbidity Titration.

In the practical we made up solutions of 9ml using different volumes of acid and distilled water. Here are 3 examples; it's been a long time since I've done this and would really appreciate help with the method.
                              A         B           C
Distilled Water      8.40      7.75      6.50
0.01M Acetic acid  0.60      1.25      2.50

PKa = 4.76

My initial reaction was to get the Ka via the Pka and then using that to get the [H+] that can then be used to get the pH but that method would give me the same answer for all of them? Any help would be greatly appreciated.

[Borek]

Each prepared sample has a different concentration of acetic acid.

[Yordanka79]

Hi, I have the same topic and I need help. How to estimate Isoelectric Point of Casein by pH /Turbidity Titration.