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Topic: Microscale flash column  (Read 4800 times)

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Offline mir

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Microscale flash column
« on: November 03, 2006, 06:04:07 PM »
Have you tried using this method before?

http://orgchem.colorado.edu/hndbksupport/colchrom/colchromprocmicroflash.html

How large amounts of sample does it take without being overloaded?

Perhaps this would be a great idea doing before running a larger scale flash column?

You might save some time and money.
No single thing abides, but all things flow.
Fragment to fragment clings, and thus they grow
Until we know and name them.
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The things we know.
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Offline wzfan

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Re: Microscale flash column
« Reply #1 on: November 04, 2006, 04:54:21 AM »
Than ::)ks!

Offline mir

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Re: Microscale flash column
« Reply #2 on: November 04, 2006, 09:09:57 AM »
No problem  ;)

And I wonder if this might be an alternative to preparative TLC?

« Last Edit: November 04, 2006, 10:28:43 AM by mir »
No single thing abides, but all things flow.
Fragment to fragment clings, and thus they grow
Until we know and name them.
Then by degrees they change and are no more
The things we know.
- Titus Lucretius Carus

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Offline movies

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Re: Microscale flash column
« Reply #3 on: November 05, 2006, 03:55:43 PM »
I don't use this method, I prefer prep TLC, but many of my coworkers use this method a lot and really swear by it.

Offline mir

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Re: Microscale flash column
« Reply #4 on: November 06, 2006, 06:00:59 AM »
I don't use this method, I prefer prep TLC, but many of my coworkers use this method a lot and really swear by it.

Are they using the exact same method? I think Im going to try the method, but with nitrogenpressure and maybe invent some kind of a solvent container on the top of the pipette. Any suggestions to how to do that?  ::)
No single thing abides, but all things flow.
Fragment to fragment clings, and thus they grow
Until we know and name them.
Then by degrees they change and are no more
The things we know.
- Titus Lucretius Carus

http://www.ife.no

Offline movies

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Re: Microscale flash column
« Reply #5 on: November 06, 2006, 12:50:32 PM »
Pretty much just like the link you posted, I think.  The solvent reservoir at the top probably isn't necessary because the flow should be pretty fast.  Just keep a beaker with your eluent nearby and you can refill as you go.  We usually take one of those interlocking plastic tubing connecters and use that to force pressure on the pipette, usually the smaller side is a perfect fit.  You'll have to hold it there while you elute, but it's pretty easy and still quick.

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