I think 3 or 6% H2O2 will oxidize thiols to disulfide. When you boil an egg then its denaturation is because the same oxidation reactions of thiol residues(i cant remember the aminoacid thiol come from) forming cross-linking disulfide bridge structure...Do someone know if someother has worked on renaturation study of the alredy boiled eggs
I'll back to secondary amine synthesis sugesting Mannich type reactions on primary amine, formaldehyde and enolizable carbonyl compound with next Kishner-Wolf reduction of the C=O group in the alkyl residue..Also Kabachnik-Fields reaction:
R-NH2 + R1CHO(or R1COR2) + HPO(OR)2-->RNHCHR1PO(OR)2
Can someone tell us how further to remove the PO(OR)2 group or eventualy PO(OH)2 one