[mobfesk2]

Hi,

I have a sugar mixture in which sialic acid is bound to lactose + some unreacted lactose in there. I want to determine how much unreacted lactose I have in this mixture so came across the phenol-sulfuric acid assay. I'm a bit confused on how this actually works....

So if I make standards of lactose and run the assay on my sample, won't it determine all lactose.... even the ones bound to sialic acid? I only want to determine unreacted lactose..

I hope someone can help

Thank you very much