[beby]

I want to know the easiest and simplest method to extract flavonoids or more specifically flavonones in pomelo peel and pomelo callus culture. Hope anybody can help me urgently. Thank you.

[Alpha-Omega]

The USDA website has a number of areas that may be useful to you.  You will have to do a literature search.  I have found the following links that may be of use to you.  Flavanones are also addressed by USDA.

USDA Portal:  http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1OB?navtype=MA&navid=HOME

USDA research and Science:  http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1OB?navtype=SU&navid=RESEARCH_SCIENCE

This is a paper on from USDA dealing with Extraction Methods for Flavanoids:

http://www.ars.usda.gov/SP2UserFiles/Place/12354500/Articles/JAFC54_9966-9977Flavonoid.pdf

[beby]

thank you for your info, i've read it before. the other question is can i use extraction method of flavonoid and mobile phase of HPLC detection from different source (different journal)? 

[JGK]

thank you for your info, i've read it before. the other question is can i use extraction method of flavonoid and mobile phase of HPLC detection from different source (different journal)? 

You could, but dont assume it will work before testing it.  Spike test samples and take them through the procedure to check its efficiency,

[Arkcon]

A-O:'s links are free.  If you have another journal article, you can try to compare the ones you have for effectiveness, JGK: said.  But people aren't going to often reinvent the wheel for biological extraction.  If they do, they might prefer to keep it a trade secret.  If you've got a specific problem, you could mention it, and someone may have insights.