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Topic: HPLC column pressure  (Read 5909 times)

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Offline chen.1406

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HPLC column pressure
« on: March 13, 2008, 05:11:05 PM »
I am conducting HPLC, I found the column pressure is going down. Why?
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Offline Arkcon

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Re: HPLC column pressure
« Reply #1 on: March 13, 2008, 05:59:51 PM »
You're dissolving a plug, or flushing out something with a higher viscosity, either a plug of sample, storage solvent or a gradient.  Or you have a leak.
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Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline SemiAuto

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Re: HPLC column pressure
« Reply #2 on: March 16, 2008, 12:14:32 AM »
If you don't have a leak somewhere, maybe your column temp has changed or your pump seals are shot.
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Offline chiralic

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Re: HPLC column pressure
« Reply #3 on: March 16, 2008, 12:28:50 AM »
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Offline coquim

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Re: HPLC column pressure
« Reply #4 on: March 17, 2008, 09:19:40 AM »
if you have leak, an alarm should be start in order to notice you the pressure is under a certain level and probably you´ll find a liquid in some part of the equipment. You must choice a minimun and higher level before start running the hplc. But let us know how much and in what condition the pressure is going down. There are several causes for that.
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