[Telamond]

So after going through all the different chromatography (AC, IEX, HIC, GF, RPC etc.) my lector went through ways to combine the methods and why it was logical to for an example have GF (desalting) -> AC -> GF for high salt concentrations whilst if you started with a HIC in high salt concentration you didn't need to do a GF...

The thing I didn't understand however was why HIC -> IEX was better for high salt concentration whilst IEX -> HIC was better for diluted solvents...

Also how would you be able to find out the tert. structure of a protein?

[DrCMS]

Try this link:

http://wolfson.huji.ac.il/purification/PDF/Others/AMERSHAM_ProteinPurificGuide.pdf

[Telamond]

Just what I needed!
Thank you for the *delete me*
^ what's with the *delete me*
won't let me write h e l p O.o