Dear All,
I would like to know how to prepare a 1 Litre buffer of pH 7.4 and strength 100mM, using NaHCO3 and Na2H2P2O7 or other acidic sodium phosphates.
It is important that the ingredients are all solids, but soluble in water at 25 deg C and the pH of 7.4 should be maintained even when the 1 Litre buffer is diluted to 100 Litres.
Thanks in advance,
jmarjorie