[rock candy]

i think im sleepy or confused cuz i dont even understand what im suppose to do

i have to make a standard curve using a 1mg/ml sample of BSA

i have to place 3 ml of Bradford reagent into 8 test tubes

but only 5 of the 8 tubes are going to be used to construct the curve , while 2 will contain 2 unknowns

--- im okay up to here ( its like what would the last test tube be used for , im gonna say a blank so that i could calibrate it based on the Bradford reagent )

i think i forgot how to do these stupid calculations tbh.. its like int he 5 standard test tubes , place 2, 5, 10, 25 and 50 microliters of 1mg/ml of BSA

then it goes on rambling bout stuff i know... am i stupid or something cuz i think im just suppose to convert to ml and then do c1v1=c2v2

[Yggdrasil]

Here's what you generally do to construct a standard curve.

1)  Figure out the amount (mg) of BSA added to each of the standards.

2)  Plot the absorbance (y-axis) versus mg of BSA (x-axis) and determine the best fit line.

This best fit line is your standard curve.  You can use this to convert the absorbance of your unknowns into a mass of protein in order to figure out the concentration of your unknowns.