[lakersfan]

What happens during the process of conjugating BSA to oligos and BSA to SANH?  In other words, what is the science behind it?



Okay, so I'm doing a research report.  I followed a protocol for the modification of BSA.  I want to see if conjugating BSA to oligos will increase the sensitivity of the results in my DNA microarray chip.  So far, I conjugated BSA to oligos powder and also conjugated BSA to SANH.  After running a protein gel, I saw that the well that BSA conjugated to the oligos shifted upwards compared to the well that had BSA conjugated to SANH.  Therefore, I can see that the oligos and the BSA did bind.  Hopefully, this will increase the sensitivity of my results when I conjugate BSA to oligos on the DNA chip.  (By the way, we are designing DNA chips for efficiently genotyping HPV strains.)

 

Thank you for your input!