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Topic: proper staining technique.  (Read 6435 times)

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Offline 8888kirbz

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proper staining technique.
« on: November 23, 2009, 07:07:28 PM »
What is the proper procedure in staining blood smears with acridine orange?
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Offline JGK

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Re: proper staining technique.
« Reply #1 on: November 24, 2009, 12:10:04 PM »
Check the bottom of Page one [url][http://www.pmlmicro.com/assets/TDS/885.pdf/url]
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Offline 8888kirbz

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Re: proper staining technique.
« Reply #2 on: November 27, 2009, 08:18:31 AM »
that's exactly what i've done sir... but some of my slides having blood smears has its cells not so clearly stained. I dont know what went wrong. Will it be ok if i'll jus restain those slides?
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Offline JGK

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Re: proper staining technique.
« Reply #3 on: November 27, 2009, 03:40:51 PM »
To be honest I wouldn't know the last time I satined blood smears was in the 1980s (using H&E stain).

However, I wouldn't recommend restaining all of the slides at once. Try one or two first to see if they will take more stain.
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