[MissDee]

Hi,

I'm stuck on a few questions related to biochem.

1.) Regarding locoweed poisoning: fluorocitrate inhibits aconitase, which is an enzyme that catalyzes a reaction in the citric acid cycle. How can aconitase inhibition be treated?

2.) Beat oxidtation of fats is inhibited by acetyl CoA, the product of the spiral. What type of inhibition is this? This type of inhibition is an effective means of regulation of this and other pathways and cycles, how? -. Is this referring to positive and negative feedback?

3.) How does body temperature increase, respiratory rate/oxygen update, weakness, sweating, body weight decrease related in terms of the electron transport system and oxidative phosphorylation with uncouplers? -...I understand that weight reduction is due to the dissipation of energy as heat, like that found in dinitrophenol. But how do the rest of these symptoms result from ETS/oxidative phosphorylation?

4.) Thermogenin. When a fatty acid is bound to thermogenin, protons are allowed to return across the membrane. What type of process is this? Will ATP be produced? Will the animal with termogenin maintain its weight?

5.)

Acetyl CoA --> mevalonate -(enzyme HMG CoA reductase)-> isoprene --> squalene --> cholesterol

Which metabolites in the pathway will accumulate? Which metabolites will decrease?


6.) Unsaturated fatty acids have what orientation around the double bond? Cis, trans, unsaturated, or cis & trans?

7.) A single cycle of the citric acid cycle produces how many ATP in substrate level phosphorylation?... Is it 2 ATP?



Any help is appreciated, thanks!