January 06, 2025, 10:53:27 PM
Forum Rules: Read This Before Posting


« previous next »
Pages: [1]   Go Down

Topic: Why should IPTG induction be carried out in the dark?  (Read 5647 times)

0 Members and 1 Guest are viewing this topic.

Offline kristinase

  • New Member
  • **
  • Posts: 4
  • Mole Snacks: +0/-0
Why should IPTG induction be carried out in the dark?
« on: August 13, 2010, 12:16:54 PM »
In several protocols for protein expression in recombinant E coli with IPTG induction they put the cultures in the dark after induction and I was just wondering why this is done. Thanks!
Logged

Offline Wreath

  • Regular Member
  • ***
  • Posts: 38
  • Mole Snacks: +1/-0
  • Gender: Male
Re: Why should IPTG induction be carried out in the dark?
« Reply #1 on: August 15, 2010, 05:09:01 PM »
because IPTG is degraded by light.
Logged
Pages: [1]   Go Up
« previous next »
Sponsored Links