[Bohemian]

Ethanol + NAD⁺ Acetaldehyde + NADH + H⁺.

You have 1ml solution containing 100uM of Ethanol and 1mM of NAD⁺. You then add 1mg of alcohol dehydrogenase. Assuming all of the ethanol is converted to acetaldehyde, what will the final concentration of NADH be in the solution?

    I understand the reaction and how ADH catalyzes it. Normally, I have no issues determining concentrations. That is general chemistry stuff. This one has me a bit confused though. Every which way I do it, I end up with too many grams and a concentration too high. I feel like I'm missing units here, particularly as pertains to volume. I would greatly appreciate some help.

   I require the concentration so that I can plug it into the Beer-Lambert equation to find out an absorbance. That, I'm fine with. It's just this concentration question that is getting my goat.

[Borek]

You don't have to calculate anything - just look at the reaction equation. How many moles of NADH are produced per each mole of ethanol? Volume is constant.