[sciencegirl]

Hello,

I'm have the following question I'm stuck on:

A known mixture of Compound X (1.52 mg/mL) and Compound Y (1.73 mg/mL) gave peak areas of 16.02 and 9.41 cm2, respectively, after separation by isocratic HPLC.  A solution was prepared by mixing 8.76 mg of Compound Y with 5.00 mL of unknown containing just Compound X, and diluting to 10.00 mL.  Peak areas of 5.96 cm2 (Compound X) and 4.86 cm2 (Compound Y) were measured.  Find the concentration of Compound X in the unknown.

I'm not sure where to start.  Can anyone give me a rough sketch of how to do / approach this problem?

Thanks!

[Pradeep]

First you have to assume that, both compounds X and Y have smiler chemical properties, and therefore the same response factor on HPLC detector. Then you can calculate the response factor for Y and then apply that on X. Then calculate the X content.