[samuel]

My question is if the following method is good for isolaiting clorophyll without damaging his internal structure (and wthout removing the Mg²⁺ from it):
1)Crushing spinach leaves in a 96%Ethanol solution.
2)Filter the solution to remove solid residues
3)Put the solution into a small matrass and make the pressure decrease with an external pump
4)Heat the matrass about 40-50ºC until all ethanol evaporates while the pump is working.
*The points 3-4 are something like using a "rotavapor" so that i wouldn't have to heat too much in order to don't change clorophill structure.

Is this a good method?Would you change something? (or if there's a better one pleas tell me, but I have to do that
without using strange lab tools)

[Honclbrif]

Its not a bad way to start. How pure do you need your chlorophyll? Using this method you will get a crude extract that also contains many other chemicals from the plant (carotenoids, etc...)

[samuel]

Hi,
There's no problem on containing carotenoids, xanthofills etc. In fact the only condition is that the extract doesn't contain anything poisonous for a plant.
Ah, wich temperature do you think that is the maximum that chlorophyll can support?
Thank you
PS: I have just ended my first organic chemistry subject, so I don't know many things about organic chemistry.

[im_newbie]

hi,

i want to isolation secondary metabolite (flavonoids, alcaloids, etc), but without the chlorophyl. so i want to get pure of them. is there anyone know how to do that ?