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Topic: Negative transmission value using UV-vis spectroscopy  (Read 4803 times)

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Offline NormalPolymer

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Negative transmission value using UV-vis spectroscopy
« on: July 31, 2011, 05:47:07 PM »
Hi, all:

I'm using a UV-vis spectroscope (Lambda-900, PerkinElmer) for a transmission measurement. I autozero the baseline from 300 to 700 nm using two parallel polarizers, and then set wavelength=0 nm so that the probing light is white light. By rotating the second polarizer, I should be able to get maximum when two polarizers are parallel and minimum when two are perpendicular. Now I have a problem: I got a negative transmission value when two polarizers are perpendicular.
What could cause a negative T value, and what can I do to solve this problem?
Thank you all!


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