[Henderson-Hasselbalch]

Hi,
For my environmental chemistry paper I needed to prepare a calibration curve for an experiment. I plotted the absorbances in EXCEL, obtained the slope and the slope's error. Does the error of the slope encompass the uncertainty associated with the dilutions of the stock solution to make the calibration standards (from micropipettes, volumetric flasks, etc.)?

[voidSetup]

I think so, it's a different kind if uncertainty from a distribution of measurements vs an uncertainty associated with an instrument or tool.

[Stepan]

How you build your standards and plotted the calibration line or curve this is up to you. Uncertainty is measured as standard deviation of matrix spikes prepared from independent stock and measured 7-10 times at different loads: at the detection limit, at the middle of you range and near the upper edge of the range.

Properly calibrated pipettes add only 0.5-1% of uncertainty into overall error.