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Topic: how to separate two water soluble compounds with diff retention times in HPLC?  (Read 5922 times)

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Offline Chaste

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how to separate two water soluble compounds with diff retention times in HPLC?
« on: September 05, 2011, 10:46:58 AM »
Hi all,

as above. are there any other methods beside using liquid chromatography?
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Offline Stepan

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Re: how to separate two water soluble compounds with diff retention times in HPLC?
« Reply #1 on: September 05, 2011, 02:01:45 PM »
HPLC is the most expensive among all. LC is way cheaper, TLC even less expensive. Distillation, re-crystallization, re-precipitation, you name it 
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Offline Chaste

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Re: how to separate two water soluble compounds with diff retention times in HPLC?
« Reply #2 on: September 06, 2011, 12:45:52 PM »
hi Stepan,

how exactly would you use TLC? can you give me some pointers please? just wanna add that these 2 compounds are unknown.
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Offline Dan

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Re: how to separate two water soluble compounds with diff retention times in HPLC?
« Reply #3 on: September 06, 2011, 12:55:48 PM »
Here is an example. This was the 5th hit on google for preparative TLC.
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Offline Stepan

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Re: how to separate two water soluble compounds with diff retention times in HPLC?
« Reply #4 on: September 06, 2011, 06:44:02 PM »
Thank you Dan. Basically you separate your compounds on the plate, then scrape the spot with you compound off the plate, and extract your compound with appropriate solvent.
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