[Kaladiscope]

Hi,

thats the thing, how to get rid of the buffer. I have performed a reaction and I would like to remove the buffer. Any suggestion about the methods to do that?

Thanks

[Arkcon]

When we used TRIS buffer for a chromatographic purification of a large biomolecule, and had to suspend the final product in another buffer, we used dialysis (actually diafiltration.)  This is time consuming.  I assume you used TRIS for a similar application, not an organic synthesis, because if that were the case, maybe you can solvent extract out the product?

[Kaladiscope]

Well I dont think that extraction with any organic solvent would allow me to remove tris buffer since my compounds have more affinity to water than any organic solvent.

I have performed the reaction in tris buffer and right now I would like to remove it....i am trying to find something in the literature but I can not find anything...

[Babcock_Hall]

If your reaction involves a large molecule, such as a protein or nucleic acid, then gel filtration should easily allow one to remove the buffer.  There are commerically prepared columns, or one can pour one's own.

[Babcock_Hall]

If your compound is neutral, it might be possible to remove the tris buffer with some form of ion exchange, possibly mixed-bed.