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Hi all,
 I have been trying for some time to try and bind small molecules to DNA using fluorescence properties of small ligands thereby allowing for calculation of association constants using Scatchard/McGhee Von Hippel analysis.  I've read a great deal of experiements, and have performed a good number of titrations with DNA & ligand at varying concentrations.

I was hoping someone here has experience with these kinds of experiements and could give me some pointers.

1)  How do you choose a good concentration at which to do the studies? [both concentration of ligand, and of the DNA]
2) Is there any place someone can point me to, that would tell me how to perform a McGhee von Hippel Analysis to determine the number of base pairs a small molecule would bind to using fluorescence experiments and find a per base pair association constant? [using specifically fluorescence techniques]

Thank you very much.