November 26, 2024, 05:37:14 PM
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Topic: Reaction of crystal violet (oxidised form) with 3-aminophthalic acid.  (Read 1686 times)

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Offline PFS

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Colleagues,
I have a fluorescent enhancement (fluorophore) effect when 3-aminophthalic acid is added to a dry, dilute residue of crystal violet. The Emission spectrum maxima shifts to a higher wavelength with increased intensity compared to that of the EM spectrum of CV.
Based on fluorescence characterisitics, this reaction product seems stable under lab conditions. 
Would anybody be able to assist with proposing a reaction mechanism and product for this reaction?
Background Info:
The 3-aminophthalic acid is the product form of luminol (5-Amino-2,3-dihydro-1,4-phthalazinedione) after it reacts (via a catalyst such as the haemoglobin (Fe2+) in blood) with a suitable oxidising agent - usually H2O2. The reaction also produces energy in the form of chemiluminescence which is why luminol is used as a presumtive test for the presence of blood. Undesirably,the chemiluminescence is short lived.
I welcome and appreciate your  knowledge and thoughts on this topic.

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