[Nescafe]

Hi,

Does anyone know why fluorescent is enhanced when certain dyes interchelate with DNA? How does stacking with the DNA base pair exactly effect the electronics of the dye that leads to enhanced fluorescence?

:S

Thanks,

Nescafe.

[Arkcon]

The  intercalating dyes (interchelating would be a different thing entirely, assuming it exists) such as ethidium bromide have florescence enhancement with double stranded DNA by a number of mechanisms, outlined here: http://en.wikipedia.org/wiki/Ethidium_bromide#Structure.2C_chemistry.2C_fluorescence