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Topic: GC Analysis Lab (Read 2654 times)
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ahhppull
Regular Member
Posts: 21
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GC Analysis Lab
«
on:
November 25, 2012, 11:02:59 AM »
How do I find the concentration and response factor of each compound using the retention time and the area?
My data is
Retention Time Peak Area
1.850 4489.8740
3.266 8536.9520
4.933 10534.9770
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curiouscat
Chemist
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Re: GC Analysis Lab
«
Reply #1 on:
November 25, 2012, 11:06:10 AM »
All you can do with this meagre data is
% conc. = area / [ sum of areas ]
But won't be very accurate at all.
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ahhppull
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Posts: 21
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Re: GC Analysis Lab
«
Reply #2 on:
November 25, 2012, 11:09:34 AM »
Also for this standard, I got the percentage of each compound. Would this help? Octace (1.15%), Nonane(1.91%) and Decane (2.24%)
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sschoe2
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Re: GC Analysis Lab
«
Reply #3 on:
February 16, 2013, 08:34:48 PM »
From the data you have the best you can do is a percent report which as best is an estimate for most detectors. For the FID similar compounds often have similar response factors. For MSD and others not really. Also keep in mind you can only see volatile compounds.
For an accurate quantitation you need a reference compound and calibration curve. Without a method development the only good way to do it would be standard addition. Otherwise you have no idea what effect the matrix has ie the recovery.
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