[jey]

Hi..i need to answer a question for my lab report on lysozyme activity and i don't know how.
This is the question..On the basis of the evidence you now have, can you computate the % purity of your most active fraction?(100% means no other protein present). Explain.
These are the data I've got..
                                Volume, mL
Raw material (egg white)   16.03 g
Sample A                               51
Sample B                               42
Sample C                                9

Sample/Volume, mL/Activity, U mL-1/Total Activity, U/Protein Concentration, µg mL-1/Protein Amount, µg   
A            51         103                  5253                      7436                            379236   
B            42         17                   714                      3918                             164556   
C            9         117                  1053                      120                                 1080   
         

          Specificity Activity, U mg-1
A                13.85
B                 4.34
C                 975
   
Is % purity and % yield similar? Can i find for % yield and the answer minus with 100% to get the % purity?
Please help me with the calculation!         

[Arkcon]

Its hard to follow your table data, what the units headings of the table are, and what your various definitions are.  However, here's something you can do to help you figure it out:  You've conducted (I assume) two assays, one for total protein, and one for lysozyme activity.   By (your) definition, 100 percent purity would be some activity level (I can't figure this out from your units headings, sorry) with such low levels of total protein that the fraction is 100% -- so practice that -- make up some data, and calculate with it, until you get 100%.  Then use the real data in the calculation you've determined.

[Babcock_Hall]

Purity and yield (recovery) are orthogonal.  You could have high purity and high yield, or any other combination of the two.  One assesses them differently.  Recovery can be judged by how many units of enzyme activity you started with versus how many units of enzyme activity you recovered in the end.  I agree with Arkcon that your table headings don't provide as much information as they should.

[Babcock_Hall]

Do you know the definition of, and the utility of, specific activity?  That is a critical point IMO.