[GoGoGadget]

I am trying to figure out whether if imprecise pipetting of an enzyme of which you are trying to determine its Km and Vmax become affected or not or which do? I'm not sure how you would be able to find this out...

[Archer]

Could you please provide your understanding of what K_m and V_max relate to.

[Babcock_Hall]

It might also be worth asking what factors affect the rates of enzyme-catalyzed reactions in general.  You must show an attempt to solve the problem first; that is a forum rule.

[micromelissa]

I could imagine that improper technique could result in different Km and Vmax values. Afterall, isn't the rate of the reaction dependent on the substrate concentration? (improper pipetting could alter your concentration). I just asked a question on Enzyme Kinetics in the forum. You should take a look at it. Hope that helped. Thanks..

[Archer]

I could imagine that improper technique could result in different Km and Vmax values. Afterall, isn't the rate of the reaction dependent on the substrate concentration? (improper pipetting could alter your concentration). I just asked a question on Enzyme Kinetics in the forum. You should take a look at it. Hope that helped. Thanks..

Please read the two previous responses to this thread.

 We haven't provided the answer, not because we don't know but because it is better to have the poster work it out for themselves through prompting them to think rather than simply providing an explanation immediately which they can cut and paste into their assignment.

[Babcock_Hall]

I could imagine that improper technique could result in different Km and Vmax values. Afterall, isn't the rate of the reaction dependent on the substrate concentration? (improper pipetting could alter your concentration). I just asked a question on Enzyme Kinetics in the forum. You should take a look at it. Hope that helped. Thanks..

But your initial post is not about pipetting substrate; it is about pipetting enzyme.