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Topic: Murexide Test and Test for Phosphate  (Read 3335 times)

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Offline dystopiaxx

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Murexide Test and Test for Phosphate
« on: February 23, 2014, 07:39:54 AM »
The topic for our class is the Characterization of Nucleic Acids. What would the equation of the reactions of the Murexide test be with guanine and adenine? Everything I find starts off with uric acid, and I'm not happy to admit this, but I don't understand it at all because I find Chemistry extremely difficult. :( Also, what would the equation of the reaction of phosphoric acid be with the test for phosphate?

Murexide Test, according to my school's lab manual:
+ 10 drops/1 microspatula of test compound
+ 5 drops concentrated HNO3
evaporate to dryness over water bath
moisten residue with 10% KOH
warm over water bath
+ 10 drops warm water

Test for Phosphate, according to my school's lab manual:
+20 drops of test compound
+ 5 mL hot distilled water
+ 10 drops concentrated HNO3
+ 20 drops 2.5% ammonium molybdate solution
warm in water bath and let stand for 10 minutes

Thank you very very much for reading this! Idk why the word "h e l p" turns into "*delete me*", but thank you for that too.

Also, if I posted in the wrong forum, I'm sorry. As I said, I am a horrible Chemistry student. :( I'm taking steps to fix it though and I really want to learn. :(

Offline billnotgatez

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Re: Murexide Test and Test for Phosphate
« Reply #1 on: February 23, 2014, 11:03:22 PM »
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Thank you very very much for reading this! Idk why the word "h e l p" turns into "*delete me*", but thank you for that too.

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