Hello everyone,
I'm trying to measure the pka of a small molecule in water. I dissolved some of this molecule in a sodium phosphate buffer solution of pH 7.3. and proceeded to adding HCl 0.1 M. I obtained asigmoid curve the point of inflection of which occurs at pH 4.6. Can I say that 4.6 is the pKa of my molecule is 4.6? Can you titrate pKa in a buffered solution? Should I redo my experiment in distilled water?
Thanks!