Your question is a little hard to follow, and I'd like you to work on these problems first, before we get to your real question.
You have to use significant figures intelligently. No way in hell are six figure optical uinits in any way correlateable to masses of actives humans can measure on an analytical balance, and they are in no way statistically significant to any animal model. So stop doing that, right now, because it just looks silly.
I don't recognize your units -- "mAU min", maybe you mean "mAU/min" or some other missing punctuation. You should mention your instrument -- is this some sort of kinetic assay? Let us know, so an expert can find this thread, and help you out. You mention calibration area -- is this an HPLC or GC method? In that case, we tend to identify such a method, and just call it peak area, without mentioning the chromatogram's units.
But briefly, no, if you want to evaluate an unknown, you have to be with a recently calibrated range, or be using a previously validated method known to cover that range. But I can't read your numbers, so I have to take you at face value that you're outside the range.