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Topic: Analytical Chem  (Read 2360 times)

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Offline anita-573

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Analytical Chem
« on: December 24, 2015, 07:48:06 PM »
Hi, any help would be greatly appreciated as I have no idea how to begin, the answer is meant to be 1.27 mg mL-1.

The concentration of iron in a blood sample was determined by
flame atomic absorption spectrophotometry as follows: 2 mL of blood was
extracted with 20 mL of methanol and filtered. 1 mL of the extract was diluted to
20 mL with methanol and aspirated onto the flame. The absorbance was 0.115
(arbitrary units). A 10 mg/L standard iron solution yielded a signal of 0.180 under
the same conditions. Calculate the quantity of iron in the sample in mg mL-1

Offline Borek

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Re: Analytical Chem
« Reply #1 on: December 25, 2015, 03:25:14 AM »
Hint: how does the signal depend on the iron concentration in the solution that is being feed into the flame?
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Offline anita-573

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Re: Analytical Chem
« Reply #2 on: December 25, 2015, 03:37:01 PM »
so the change in the signal equals the change in iron concentration.

Offline Borek

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Re: Analytical Chem
« Reply #3 on: December 26, 2015, 03:01:14 AM »
so the change in the signal equals the change in iron concentration.

No, it doesn't "equal" the change. It reflects the change.
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