[vahorat4]

Pectin estimation of Flax stems using D-GLUCURONIC ACID & D-GALACTURONIC ACID (D-GLUCURONATE & D-GALACTURONATE) ASSAY PROCEDURE K-URONIC 08/18?

Can anyone please help me figure out the procedure or steps to follow from the K-Uronic kit for the estimation of pectin for fibrous plant material (in this case - flax stems)? I know I am asking a question that has an answer in it, but it is confusing if i should follow some other steps mentioned (such as fat removal from plant material on page 9 - step f) in the guide or not.
Thank you for your help
The document is available for download on megazyme website: https://secure.megazyme.com/files/Booklet/K-URONIC_DATA.pdf
Flax