November 27, 2024, 08:32:29 AM
Forum Rules: Read This Before Posting


« previous next »
Pages: [1]   Go Down

Topic: chem question  (Read 4620 times)

0 Members and 1 Guest are viewing this topic.

NL1982

  • Guest
chem question
« on: August 17, 2005, 08:51:54 PM »
hi, im a newbie at this website, and a first year biochemistry student, and i am having a difficulty in regards to the following question

"a reaction is designed to be carried out ina 5 ml total volume. It requires a a final NAD concentration of 6mM. If the protocol allows 90 ul for the NAD addition, what concentration would you make up the stock solution?"

if someone could guide me in to answering this question, that would be great. Im not a genius so please don't make the answer sound complicated.

Cheers
Dave
Logged

Offline Donaldson Tan

  • Editor, New Asia Republic
  • Retired Staff
  • Sr. Member
  • *
  • Posts: 3177
  • Mole Snacks: +261/-13
  • Gender: Male
    • New Asia Republic
Re:chem question
« Reply #1 on: August 18, 2005, 05:02:47 AM »
Quote
If the protocol allows 90 ul for the NAD addition

i don't recognise the unit "ul". what is that?
Logged
"Say you're in a [chemical] plant and there's a snake on the floor. What are you going to do? Call a consultant? Get a meeting together to talk about which color is the snake? Employees should do one thing: walk over there and you step on the friggin� snake." - Jean-Pierre Garnier, CEO of Glaxosmithkline, June 2006

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27862
  • Mole Snacks: +1813/-412
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re:chem question
« Reply #2 on: August 18, 2005, 05:12:19 AM »
Quote from: geodome on August 18, 2005, 05:02:47 AM
i don't recognise the unit "ul". what is that?

u often stands for greek ? (mi, 10-6). There is no ? on keyboard and most peaople doesn't know how to enter it. And even if they know, it is often diplayed as some bush.
Logged
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27862
  • Mole Snacks: +1813/-412
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re:chem question
« Reply #3 on: August 18, 2005, 05:14:59 AM »
Quote from: NL1982 on August 17, 2005, 08:51:54 PM
a reaction is designed to be carried out ina 5 ml total volume. It requires a a final NAD concentration of 6mM. If the protocol allows 90 ul for the NAD addition, what concentration would you make up the stock solution?

1. Calculate amount of NAD that have to be added to 5mL to obtain 6mM concentration.

2. The same amount of substance must be present in 90 ?L of added solution.
Logged
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info
Pages: [1]   Go Up
« previous next »
Sponsored Links