[topenator]

I have a yam tuber sample which I freeze-dried and macerated with 95% ethanol, then concentrating it using a rotary evaporator and dissolving in 5% acetic acid. I got an acid supernatant which I filtered and made alkaline by using ammonium hydroxide then extracting it with DCM using a separatory funnel. I used the rotary evaporator again by using the combined DCM extract and used a small amount of absolute ethanol. Then I used a saturated picric acid in water and it formed a yellow picrate. I made it alkaline again by using ammonium hydroxide followed by extraction with DCM. My question is, I was supposed to use acetone: water: ammonia (90:7:10) as eluent for my silica gel column chromatography to further purify my desired alkaloid (Dioscorin) but I can't for a bunch of reasons. What alternative eluents can i use?

[Babcock_Hall]

Do  you mean discorine?  What is your intended purpose?

[topenator]

I want to purify my sample extract by performing a silica gel column chromatography, but the eluents (acetone and ammonia) I was supposed to use cannot be acquired for reasons. What alternative eluent in CC can I use to further purify the alkaloid?

[wildfyr]

This is weird. You have a rotovap, DCM, picric acid, and a column but you can't get acetone and ammonia? Those are some of the easiest chemicals to acquire for anyone, let alone someone doing formal science.

And you're purifying a toxin. Explain yourself please.

[topenator]

I'm an undergraduate student, acetone & ammonia can't be purchased freely in my country. I'm just finding solutions and alternative chemicals to use in my column chromatography to further purify my toxin (which I will extract) to test its antimicrobial activities..