[Chaggy]

Can you please help me with this tabe? thanks

[Babcock_Hall]

It is a forum rule that you must show your attempt before we can help you.  Is the guanidinium chloride becoming more dilute?  You may have learned a simple formula for this.  In my experience the math is straightforward, but it is also easy to misapply the formula.

[Chaggy]

I did not show the attempt because it is actually the first time I come across a question about diluting a mixture that consist of 3 solutions. I have calculated dilution of 2 solution before but not 3. and it is a pre lab exercise without providing any formula it's just the table.
GuHcl is increasing, myoglobin stays 500 microlitre, and the buffer should decrease as GuHcl increases

[Babcock_Hall]

From the first row in the table, you know the final volume for all of the solutions, which will stay constant, as you imply.

[Borek]

Every dilution problem is based on the same principle: whatever is put in the solution, stays there. This is just a mass conservation. If you know final concentration and final volume, you can easily calculate amount of substance needed, this will let you calculate volume of the solution that is source of the substance.

Looking for special "formulas" almost never works. What works is understanding the logic behind, this can help solve any problem, not only the ones you have been shown previously.