[gumusonur]

I have glucose syrup on hand and it contains 60% glucose. I want to get gluconic acid by reacting with enzyme but I can't. What should the quantities be, what should the conditions be? do you have any idea?

[Borek]

Is there any procedure you are trying to follow? What have you tried, what have you googled and found so far?

I can't

At this level of generality, without any details, there is really no answer that you can get from anyone.

[gumusonur]

I found different articles from google, I mixed glucose and enzyme for 24 hours at different pH values, but I could not reach a result.

[Babcock_Hall]

I assume that you are using glucose oxidase.  How are you dealing with hydrogen peroxide?

[Babcock_Hall]

post script, I would also try searching using PubMed.

[gumusonur]

thanks @Babcock_Hall

The presence of hydrogen peroxide is not a problem for me. The presence of peroxide is not a problem as long as gluconic acid is formed.

[Borek]

My car is broken, do you know how to fix it?

You are failing to give ANY relevant information, yet you expect us to help. Won't work, you are just wasting your time and our time.

[gumusonur]

Borek,

If I had any information, I would do it, I researched and couldn't do it, I asked if anyone had an idea.Do you have to give the same answer to everyone? The example you gave is irrelevant.

[Babcock_Hall]

gumusonur,

I did a little reading last week, and hydrogen peroxide is indeed a problem.  One obvious difficulty is that some enzymes are inactivated by it, and I have reason to believe that such is the case here.  PubMed is a good search tool, and I highly recommend it.  When I used gluconic acid as my search term, I found 77 review articles (there were additional articles, but reviews are sometimes a good place to start).  There are some papers that fall outside of its scope, however.

[gumusonur]

Babcock_hall

Thank you very much for your interest and support.

[Babcock_Hall]

Can you provide references to one or two papers you have found by searching?

[Borek]

If I had any information, I would do it

You said you found recipes and you did the experiment. We don't know what the recipes you found say, we don't know how you followed them, we don't know what concentrations you used, we don't know what pH you tried, we know absolutely nothing. These are all important information without which nobody will be able to even suggest anything.

[gumusonur]

Can you provide references to one or two papers you have found by searching?

Babcock_Hall

some of the ones i found

[Babcock_Hall]

https://doi.org/10.1039/D0CY00819B
"Enzyme production of D-gluconic acid and glucose oxidase: successful tales of cascade reactions"
From the abstract, "One of the main topics in this review is the problem of
the generated side product, hydrogen peroxide, as it is an enzyme-inactivating reagent. Different ways to remove
hydrogen peroxide have been used, such as metal catalysts and use of whole cells; however, the preferred method is
the coupling glucose oxidase with catalase."

[gumusonur]

thanks Babcock_Hall,

I will take into account the article you sent and make a re-evaluation.