[dune003]

I tried the DNS (3,5-Dinitrosalicylic acid) assay for my enzyme endoceullase activity analysis with beta-glucan as a substrate. I got the absorbance results. so, from the absorbance results, I don't know how to calculate enzyme activity and its specific activity.  Help me solve this problem. Can anyone help me solve this. On Monday, I need to submit my assignment.

[Babcock_Hall]

I will provide a little bit of assistance to get you started, but according to the rules of this forum, you must show your work to receive help.  In general, activity is calculated as a number in µmoles per minute.  Specific activity is calculated as a number in µmoles per minute per milligram of protein.  Notice that what is needed is an amount, not a concentration, in the numerator.  Likewise the denominator is a mass, not a concentration.  The activity of an enzyme is an extensive property, and the specific activity is an intensive property.

[Shrsh]

Please check the following article:

https://pubmed.ncbi.nlm.nih.gov/21235585/

It has a full protocol and guidance for calculating the enzyme kinetics (and measuring the inhibition). In brief, you need to use different substrate concentrations, incubate, and detect the metabolite formation.