[Spud]

I have set out to make nanoparticles following paper:
Pomegranate extract-loaded surfactant-free zein nanoparticles as a promising green approach for hepatic cancer- optimization and in vitro cytotoxicity
HERE>https://drive.google.com/file/d/1WabC2E06ejL9GA06RrR68pcNR1az90mM/view?usp=drive_link





I have Multiple Sclerosis and made this a goal to achieve before progression makes it impossible.
I am breaking the process down into small steps and would appreciate the forums help along the way.

The first step is purchasing Materials:
For this attempt, I have source for ZEIN 9010-66-6 99% purity and Ellagic Acid 95% purity.
My first question regards the amount of Zein and Ellagic Acid required?

Table 2 Preparations and characterization of PE-ZN NPs
This seems to suggest that Z12, the chosen Surfactant free mixture used equal parts PE:ZN at 0.2 (% W/W)?
Am I reading this correctly?

[Borek]

Are you doing it in an academic/industrial R&D environment?

[Spud]

I don't put limitations on what people can or should do, so kitchen environment

I am reading the paper to mean:
ZN 0.2 (% W/W) is dissolved in 5ml 70% v/v ethanolic aqueous solution.
PE 0.2 (% W/W) is dissolved in 5ml methanol.
(But still checking references because not 100 percent certain at this stage!)
For water, 0.2% (W/W) of 5ml = 10mg. But I'm not sure if this is correct.
Perhaps I am reading this wrong.

After that, the two solutions were mixed and added drop wisely into a pH-adjusted aqueous phase (10 mL) (with either 0.1 N HCl or 0.1 N NaOH) under stirring with a magnetic stirrer (AccuPlate™ Analog Hot Plate Stirrer, UK) at room temperature and 2000 rpm for 1 h until the evaporation of alcoholic solvents.
 
The mixer will have to be a cheap kitchen alternative or drill, not looked into it at this stage!

I am interested in the valve or restriction that releases drops, because this task is not simple given my experience with irrigation design and pressure compensation. The commercial solution could be easier?

[Spud]

This is my first goal, after which there are other nanoparticles of interest for H2O2 signaling in agriculture.