Hello, I am hoping someone can help me as I think this is a very easy question for those with a background in chemistry. I am hoping to produce a 1% solution of 2,3,5-Triphenyl-2H-tetrazolium chloride (98%) for the purposes of staining seeds to test for viability. Available procedures suggest dissolving 1g into 100ml of water, using water, ethanol, methanol, acetone or DMSO as a solvent.
I have tried dissolving 1 gram into 100ml of distilled water and it didn't dissolve as there were many white crystals still present. I then tried dissolving the granules in ~10 ml of 96% Isopropyl alcohol and then added 90 ml warm distilled water with the same results, many white crystals present.
The chemical is from Thomas Scientific and information is as follows:
CAS#:298-96-4
Form: solid
Formula: C19H15CIN4
Formula Weight: 334.80
Sensitivity 1: Hygroscopic
Sensitivity 2: Light sensitive
Any help would be appreciated.