[nag]

Hello to all
I wish to know the fish oils testing by HPLC for fatty acids analysis possible with UV-Vis detection.
If anybody knows please let me know the procedure.

Thanks in advance.

T.Nageshwar

[chiralic]

Hello nag:

If you have access to SPRINGER at your University, you'll must check this:
http://www.springerlink.com/content/fku6730m28v37121/

or on Taylor (Metapress)
http://taylorandfrancis.metapress.com/(egubxg45wsl5gmqvwurvkqqi)/app/home/contribution.asp?referrer=parent&backto=issue,11,17;journal,25,151;linkingpublicationresults,1:102597,1

or http://www.jstage.jst.go.jp/article/jos/53/6/53_285/_article

Regards,

Chiralic

[nag]

Thank you Chiralic for your reply.
I am unable to access these articles as I have no membership.

Thank you once again.

Regards,

Nag

[mdlhvn]

     With fatty acids, It is recommended that FID or MS are very good detection methods.
     UV-VIS, in my point of view, can hardly detect fatty acids. As you know, we must form substances or complexes that are able to absorb light in UV-VIS region at a typical wavelength. I think fatty acids in fish oils don't possess that properties.

   Hope will help you!