[mobfesk2]

Hi,

I'm having a bit of trouble calculating the percent yield of my product. Can someone please help?

I did an experiment where I wanted to produce 3-sialyllactose using fetuin (a protein) and Lactose as starting materials.

So I added 1.5g of fetuin (Mr=44 kDa) and 769mg of lactose (Mr = 342) to 15ml of 50mM phosphate buffer, I then added 1500ul of my enzyme which would transfer sialic acid from fetuin and add to lactose giving me 3-sialyllactose (Mr = 633).

Now I don't know how to calculate the percent yield of 3-siailyllactose  . Can someone please explain how to do this?

Thank you very much

[Yggdrasil]

Well, to start, how many moles of each reactant do you have?

[mobfesk2]

Ok.... so I have 2.25 x 10-3 moles of lactose (0.77g/342)

Fetuin is a protein so I think I have 3.5 x 10-5 moles of fetuin (1.5g/44000)... Maybe this is wrong?

Thanks for your reply!

[Yggdrasil]

Looks right to me.