[Bronwen Dekker]

Until the end of October, all of the protocols in Volume 2 of Nature Protocols  are freely accessible!

Here is the link to our homepage: http://www.nature.com/nprot/index.html.

I have made a list (in no particular order) below of examples that you might find interesting.


..
Synthesis and utilization of reversible and irreversible light-activated nanovalves derived from the channel protein MscL
http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.196.html

Facile synthesis of Ag nanocubes and Au nanocages
http://www.nature.com/nprot/journal/v2/n9/abs/nprot.2007.326.html


Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli
http://www.nature.com/nprot/journal/v2/n5/abs/nprot.2007.152.html

Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry
http://www.nature.com/nprot/journal/v2/n5/abs/nprot.2007.107.html

Practical and convenient synthesis of coumarins from phenols and propiolic acid
http://www.nature.com/nprot/journal/v2/n4/abs/nprot.2007.103.html

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
http://www.nature.com/nprot/journal/v2/n4/abs/nprot.2007.72.html


Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation
http://www.nature.com/nprot/journal/v2/n9/abs/nprot.2007.300.html


Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe
http://www.nature.com/nprot/journal/v2/n3/abs/nprot.2007.80.html


Parallel synthesis of peptide libraries using microwave irradiation
http://www.nature.com/nprot/journal/v2/n3/abs/nprot.2007.23.html


The use of the Ugi four-component condensation
http://www.nature.com/nprot/journal/v2/n3/abs/nprot.2007.71.html



Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures
http://www.nature.com/nprot/journal/v2/n3/abs/nprot.2007.74.html

Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings
http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.221.html


Site-specific incorporation of nitroxide spin-labels into 2'-positions of nucleic acids
http://www.nature.com/nprot/journal/v2/n8/abs/nprot.2007.273.html

Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins
http://www.nature.com/nprot/journal/v2/n8/abs/nprot.2007.275.html


Synthesis of pyrimidines by direct condensation of amides and nitriles
http://www.nature.com/nprot/journal/v2/n8/abs/nprot.2007.280.html

Synthesis and enzymatic incorporation of a fluorescent pyrimidine ribonucleotide –
http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.222.html


Immobilization of enzymes on heterofunctional epoxy supports
http://www.nature.com/nprot/journal/v2/n5/abs/nprot.2007.133.html

An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature
http://www.nature.com/nprot/journal/v2/n4/abs/nprot.2007.144.html

Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems
http://www.nature.com/nprot/journal/v2/n4/abs/nprot.2007.150.html

Crystallization of soluble proteins in vapor diffusion for x-ray crystallography –
http://www.nature.com/nprot/journal/v2/n7/abs/nprot.2007.198.html

Direct ester condensation catalyzed by bulky diarylammonium pentafluorobenzenesulfonates
http://www.nature.com/nprot/journal/v2/n7/abs/nprot.2007.254.html

Automated generation of a dihydropyrimidine compound library using microwave-assisted processing
http://www.nature.com/nprot/journal/v2/n7/abs/nprot.2007.224.html

The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions
http://www.nature.com/nprot/journal/v2/n8/full/nprot.2007.245.html