[taurean]

Guyz,

What is the good stain for TLCs?  Especially I am looking for staining amides.  Please let me know.  KMNO4 stain is not working for me.

Please suggest some good book or journal articles where I can find information about stains applicable for specific functional groups.

Thanks a lot.

[agrobert]

Depending on your amide ninhydrin is a great reagent.  It tests positive for primary and secondary amines and so heating your TLC plate can cause amide hydrolysis and result in positive tests.

http://en.wikipedia.org/wiki/Ninhydrin

[taurean]

Depending on your amide ninhydrin is a great reagent.  It tests positive for primary and secondary amines and so heating your TLC plate can cause amide hydrolysis and result in positive tests.

http://en.wikipedia.org/wiki/Ninhydrin

I just tried Ninhydrin, it also did not work.  Please suggest some other stain.

[Rico]

Hey taurean

I've had good results with using PMA-dip (phosphomolybdic acid) for esters, maybe it will work for amides too.

Recipe for PMA-dip: dissolve 10 g of PMA in 100 ml of absolute ethanol.

I hope it will work for you.

Rico

[chiralic]

Hello everyone:

Read this where you can find several Staining solutions to TLC and comments:
http://orgprepdaily.wordpress.com/2006/09/27/tlc-staining-solutions/

Regards,

Chiralic

[taurean]

Hello everyone:

Read this where you can find several Staining solutions to TLC and comments:
http://orgprepdaily.wordpress.com/2006/09/27/tlc-staining-solutions/

Regards,

Chiralic

Thanks for the excellent link.

[taurean]

Hey taurean

I've had good results with using PMA-dip (phosphomolybdic acid) for esters, maybe it will work for amides too.

Recipe for PMA-dip: dissolve 10 g of PMA in 100 ml of absolute ethanol.

I hope it will work for you.

Rico

Thanks, Rico.  I will try it out.