[sergei_DSc]

Hello everyone, I would be very grateful if someone can help me in this analysis. My concern is how to debrominate the BVO (brominate vegetable oil) in Mountain Dew for anion exchange chromatography. Instinctively I was think of using NaBH4 for reduction, but wouldnt I need a large amount (expensive) since it would preferentially reduce the sugars? Also NaBH4 would probably damage the instrument? Would I get bromide or bromate? I have searched the journals and have not found how to debrominate BVO for ion chromatography. Alternatively, measuring for the BVOs themselves using HPLC sounds more involved method-wise. Appreciate any answers, thanks..

[Arkcon]

Perhaps a good first step is using a reverse phase SPE column, so you're working with just the BVO.

[sergei_DSc]

That would work, yes, although I have to check the availability columns as I can only use what I am given. Thx

[dlzc]

... My concern is how to debrominate the BVO (brominate vegetable oil) in Mountain Dew ... I was think of using NaBH4 for reduction ... Would I get bromide or bromate?

You would have to get lots of oxygen from somewhere, in order to make bromate.  I think this would be unlikely in a "carbonated soda matrix", but you could add some calcium thiosulfate to assure no free oxygen was present.  I cannot help with the rest...