[ixi]

This is for lab.

We had to analyze our data using the classical analysis and the multivariate analysis.

For the classical analysis, i need 4 Beer's plots
I plotted the graphs, but i want to know how to I find out with the lambda max is?

thanks

[ARGOS++]

Dear ixi;

I think you mean “where” the Lambda_max is? (-if I understand correct).

There are mainly two procedures to determine the Lambda_max:  A quite insecure and a much more adequate Method.

For the insecure method you can do an intensive search if somebody has already done something similar to yours and has published a numerical value!
But if you succeed that’s very insecure anyway, because ε and Lambda_max is quite dependent on the whole measuring environment!

The more secure way will be if you “scan” for the Lambda_max a Spectrum in the interesting wavelength range, or do a manual simulation of that by make several readings on increasing wavelength.
Big Advantage: As long as you keep your later method (Solvent mixture, pH, etc.) really constant, then your values fit precisely your later Concentration determination.

To inform you about most such dependencies you may visit the page:  "Corrected Beer's Law”
The later “Fit” for the Calibration you can do inside of Excel, and also validate your Calibration too.

Hope I have been of some help to you.


Good Luck!
                    ARGOS⁺⁺