Emission spectra of tryptophan (Trp) in heated and non-heated model system was recorded at lambda (em) = 300-400 nm, lambda (ex) = 290 nm; and maximum intensity was observed at 340 nm.
Then excitation spectra was recorded at lambda (ex) = 250-320 nm, lambda (em) = 340 nm; and maximum intensity was observed at 280 nm.
We can see lower intensity in emission and excitation spectra in heated model system.
But the question is:
What else we can see from described emission and excitation spectra of Trp ? What kind of information ?
Thanks in advance !