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Offline diablo

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« on: January 22, 2009, 05:10:33 AM »

Offline sjb

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« Reply #1 on: January 22, 2009, 06:03:51 AM »
Do you have a conversion between "units" and mass?

Trouble is, in my experience biochemical "units" could be anything, from moles to activities to ...

S

Offline Yggdrasil

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« Reply #2 on: January 22, 2009, 12:05:17 PM »
In general, there is no conversion to do so.  You'd be better off directly measuring the concentration of the protein (e.g. by looking at the absorption at 280nm or using a colorimetric assay like the Bradford assay).  Note that for most of these assays to work, you need a reasonably pure sample.  If you have bought your protein from a company, never assume it is pure.  You can check the purity by a method such as SDS-PAGE.

Offline diablo

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« Reply #3 on: January 24, 2009, 03:55:33 PM »
ok, thx for your answer !

Offline JGK

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« Reply #4 on: January 29, 2009, 05:41:19 PM »
You can do it if you can find a literature value for Units/mg for your enzyme.
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Offline Yggdrasil

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« Reply #5 on: January 29, 2009, 10:14:56 PM »
You can do it if you can find a literature value for Units/mg for your enzyme.

You'd have to be careful here as the value for units/mg is highly dependent on factors like temperature and buffer conditions.

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