[f77126]

Explain "why the observation that 5-hydroxyurate formed in the urate oxidase reaction is optically active" proves that dehydroisourate anion is hydrated while bound to the enzyme

It is on urate oxidase. My biochemistry pre-lab question, I can't seem to figure out how this works.

[aHerraez]

Hi
Without going into the details of that particular reaction, the optical activity in the prodcut usually relies on the reaction intermediaries not being planar (e.g. >C=R), so the chirality is preserved.
Hope this points you in the right direction.