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Topic: Enzyme for cleaving urethane bond (ester) ?  (Read 4978 times)

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Offline diablo

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Enzyme for cleaving urethane bond (ester) ?
« on: May 12, 2009, 10:25:37 AM »
Hi folks,

i just wonder if anyone of you know an enzyme wich is capable of cleavin urethane bonds (specifc).

I was thinking about cholesterol esterase, any other suggestions ?

thx.
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Offline sjb

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Re: Enzyme for cleaving urethane bond (ester) ?
« Reply #1 on: May 12, 2009, 03:18:33 PM »
Quote from: diablo on May 12, 2009, 10:25:37 AM
Hi folks,

i just wonder if anyone of you know an enzyme wich is capable of cleavin urethane bonds (specifc).

I was thinking about cholesterol esterase, any other suggestions ?

thx.

Esterases, by definition usually act on esters. Do you mean specific cleavage of urethanes (in the presence of what?), or specific cleavage of *a* urethane?
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Offline diablo

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Re: Enzyme for cleaving urethane bond (ester) ?
« Reply #2 on: May 13, 2009, 07:42:23 AM »
Hi,

thx for your answer,
i meant specific in cleaving urethane bonds only ( I've got a protein which is modified with a chemical linker - this linker bears an urethane bond- now i'm looking for an enzyme which can cleave this bond(s) ).

 
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