Im doing a Column Chromatography: Separation and Purification of Fluorene and Fluorenone in lab. Ive been asked the following question.
Hexane is the first solvent, and dichloromethane is the second solvent used for the separation of fluorene and fluorenone. Why would it be unwise to use dichloromethane first? What would happen?
I know that hexane is the least polar eluant (solvent) but I dont now what difference it makes which goes in first? Don’t fully understand the mechanics of this procedure…I’ve never done, or seen it!