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Topic: can polysaccharide be seen by uv detector  (Read 4879 times)

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Offline HB

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can polysaccharide be seen by uv detector
« on: January 19, 2011, 03:38:04 PM »
I have extracted a polysaccharide from an algae, can it be detected with UV detector or react with any chemical compound so that it can be detected with UV detector or it is impossible
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Offline JGK

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Re: can polysaccharide be seen by uv detector
« Reply #1 on: January 20, 2011, 01:47:11 PM »
Sugars have very poor chromophores and are not detected well by UV.

Most chromatography of sugars uses either Refracive Index (RI) or evaporative light scattering (ELS) detection.
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